Reverse Transcriptases
Genscript Corporation M-MuLV Reverse Transcriptase
GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E coli strain overexpressing this construct To increase cDNA yields and get a higher percentage of longer transcripts the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity and expressed free of exogenous RNases and other nucleases The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis) making it ideal for a wide range of applications
INTACT GENOMICS INC igScript™ Reverse Transcriptase 50,000 Units (200 u/µl)
igScript™ Reverse Transcriptase (igScript RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.Applications: First strand cDNA synthesis for PCR or RT-PCR Gene expression data validation by using RT-PCRBenefits: Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. Active at temperatures up to 55°C. Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.Product Includes: igScript™ Reverse Transcriptase 10x igScript™ RT Reaction Buffer
Agilent Technologies AFFINITYSCRIPT MULT TEMP TRANS
Our AffinityScript Multiple Temperature Reverse Transcriptase is engineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature.